Monday, April 8, 2013

How to deal with the sam file without the header:


How to deal with the sam file without the header:
You can produce this with the faidx command in samtools:
samtools faidx all_sequences.fa
then use the output
samtools view -bS -t all_sequences.fai infile.sam > outfile.bam
where all_sequence.fa is the whole genome fasta file, and infile.sam is the samfile without the header.

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